Wnt3a leads to increased “cluster-like” aggregations.

<p>Representative brightfield photomicrographs of cluster cells following control or Wnt3a treatment at each day of culture. At day 1 after seeding, cluster cells are arranged predominantly as singles or doublets under both conditions. At day 2, most cells remain as singles and doublets in both conditions but longer chains are more easily observable (arrowheads; upper panels are higher magnification). In day 3 control cultures, most cells remain as singles but small clumps are occasionally observed (arrowhead). In Wnt3a-treated cultures, larger clumps are observable by day 3 (arrowheads). On day 4, large “cluster-like” cell aggregations are found throughout Wnt3a-treated cultures (arrowheads), while control cultures are mostly singles and infrequently show small cell accumulations or the occasional cell chain (arrowheads) but lack large cell accumulations. Upper panels show higher magnification images. Scale bar: 50 µm, upper panels: 12.5 µm.